Electron Microscopy Core

THE ELECTRON MICROSCOPY CORE IS OPEN!! 

<PLEASE READ OUR REOPENING PLAN THOROUGHLY BEFORE REQUESTING SERVICES>

Electron Microscopy Core (AEM), NDIIF Operating Procedures during COVID-19 outbreak
Access to the Electron Microscopy Core is subject to all NDIIF facility requirements. The following procedures are subject to change at any time. We sincerely appreciate your patience as we implement these changes.

REOPENING:
All dates below are tentative and subject to be change depending upon the guidance and leadership of UND. The cores will not be opened unless the building in which they reside in is open. If a building were to close unexpectedly, the core will also close during this time.


PHASE I – JUNE 5th- JUNE 7th
General rules: working with customers


1. All Electron Microscopy training activities are suspended until further notice.
2. During this period, no users will be permitted in the facility while instruments and facility spaces are being prepared for reopening.
3. All communication will take place by appointment via email, telephone or through online conference tools such as Zoom, Skype, Microsoft Teams or WebEx.

PHASE II – JUNE 8th – TBD following guidelines provided by UND
Disinfection of the facility will be performed by the NDIIF staff at the beginning of each day by NDIIF staff. The hours of the facility have been restricted to 6:00am-10:00pm EST Monday thru Friday.


1. Experienced trained users (to be determined by Ms. Tatyana Orlova for SEM and Dr. Maksym Zhukovskyi for TEM) will be permitted in the facility by appointment only during this period. A Certificate of Wellness must be submitted to the building manager at the building designated area immediately upon arrival into the building.


DO NOT ACCESS THE FACILITY FOR A MINIMUM OF 14 DAYS if you have been in high risk areas or had contact with confirmed COVID-19 patients.
• DO NOT ACCESS THE FACILITY if you are COVID-19 positive or otherwise sick - even if you are only experiencing mild, cold-like symptoms.
If you are experiencing symptoms or have questions regarding COVID-19, you are encouraged to call the Notre Dame hotline: 574-631-5900. https://coronavirus.nd.edu/
• If you are at risk to develop severe symptoms, you are advised to avoid the facility and have colleagues or NDIIF staff do the imaging for you.


2. The time booking should go through:
Ms. Tatyana Orlova (email: Tatyana.A.Orlova.1@nd.edu) for SEM and
Dr. Maksym Zhukovskyi (email: Maksym.Zhukovskyi.1@nd.edu) for TEM.
Request should be made in advance, at least one day prior to the planned EM work.
3. A daily calendar will be posted outside of each instrument room. If you have not been given permission to use the instrument and your name does not appear on the calendar, you MUST NOT enter the room. Contact Tatyana or Maksym if you believe that a mistake has been made on the calendar and to be granted permission to enter the room.


RULES UPON ENTRY
a. Wear a mask (user provided) at all times.
b. Personnel will wash hands immediately upon entering into the EM facility space. Where sinks are not present, hand sanitizer will be supplied.
c. Gloves (facility provided) are required when entering the facility until departure. A waste container will be provided at the facility exits for disposing gloves.
d. Minimize bringing any personal belongings into the research space. Only bring with you what is essential to your research.
e. Follow instrument specific disinfection procedure at the start of your session. Disinfecting solutions (following CDC guidance): ethanol (70% min) with a contact time of 1-2 minutes should be used and has been provided to wipe down the surfaces of countertops, door handles, light switches and computer/keyboard equipment.
f. Cover keyboards with plastic wrap. At the end of the appointment remove plastic wrap from keyboards and discard, disinfect mouse, knobs and other touched surfaces with disinfecting wipes.
g. A log placed within each room must be completed indicating that cleaning procedures have been completed by each user as well as the NDIIF staff.
h. “Check in” - Register at the entrance with your name and phone number. Signage has been posted on each door that must be filled out before entering the instrument rooms. Also the user should indicate that the room is occupied by using the slider magnet posted on the outside of the door.
i. Only ONE person in the room at a time.
- Doors must be closed while systems are in use. Don’t enter the room if you see somebody working there. The break between EM sessions should be at least 30min.
j. Follow instrument specific disinfection procedure at the end of your session. Disinfecting solutions (following CDC guidance): ethanol (70% min) with a contact time of 1-2 minutes should be used and has been provided to wipe down
the surfaces of countertops, door handles, light switches and computer/keyboard equipment.
k. This space is shared by multiple users. Please check the hall before exiting the facility so that you do not accidentally walk into another person and so that social distancing of a minimum of six feet can be maintained at all times.
l. “Check Out” - Remove your name and phone number when leaving.
m. Wash hands or use hand sanitizer before leaving the space.
- If you are using JEOL, Magellan or Helios please enter the facility through B10 room and leave the facility through the alternative door located in microscope room.
- Users can come to B10 room 15 minutes prior to their appointment to do sample preparation procedures, including plasma cleaning and/or sputter coating sputter coating.
- To minimize time in the sample preparation room the users should use their own stabs, carbon stickers, copper tape, tweezers, scissors or any other tools, necessary for sample mounting (see list of sellers at the end of the policy). Samples have to be mounted prior bringing them in the EM lab.
- At the appointment time, users may enter the corresponding EM room with all their possessions.


4. Imaging services may be provided by Tatyana Orlova or Maksym Zhukovskyi. All services must be arranged in advance via email with as much information regarding the sample as possible.
5. Services provided to users during this period will be charged the ‘assisted use’ rate.
6. If instrument issues arise during the session, please call the staff at the phone number provided. If these issues cannot be resolved with instruction over the phone, you will be asked to shut down the system responsibly. A staff member will work to restore function when it is safe to do so and the system will again be made available for use.
- If you are using JEOL, Magellan or Helios please leave the facility through the alternative door located in microscope room
- All assisted instrument operations will be conducted by the laboratory specialists, while the client presence will not be allowed.


PHASE III – ‘The New Normal’ until restrictions ease as directed by UND leadership.


1. Access to instruments by trained users will continue as described in Phase II.
2. Training will take place by appointment and will be by conducted via online training and conference tools.
3. Labs are encouraged to nominate a single “super-user” to minimize traffic through the facility.

- In case of EM related emergency please contact
Tanya Orlova [torlova@nd.edu, cell phone (574) 229-1917] for SEM/FIB and
Maksym Zhukovskyi [mzhukovs@nd.edu, cell phone (574) 220-2015] for TEM

List of sample preparation tools
1. Stubs w/o clips, sizes 12mm, 18mm, 25mm P## 16111, 16119,16144
https://www.tedpella.com/SEM_html/SEMpinmount.htm
2. Stubs with clips P316144-10
https://www.tedpella.com/SEM_html/SEMclip.htm
3. Carbon conductive tabs various diameters P## 16084-1, 16084-2, 16084-3, 16084-6
https://www.tedpella.com/SEMmisc_html/SEMadhes.htm#_16084-1
4. Copper conductive tape P## 16072, 16072-1
https://www.tedpella.com/SEMmisc_html/SEMadhes.htm#_16072
5. Pelco conductive silver paint P316062-15
https://www.tedpella.com/SEMmisc_html/SEMpaint.htm#anchor1335007

Instruments and Services

The advanced Electron Microscopy Core integrates a unique bundle of state-of-the-art FEI tools that enable expert users to attack the most complex modern research problems from micron to Angstrom spatial resolution.  Sample preparation of Biological and Soft Material samples for SEM and TEM imaging is provided by the Histology Core.  Please contact Sarah Chapman for more details.

Staff

Researchers can be trained to use our devices, or services can be provided by experienced staff.  Contact the director of electron microscopy, Dr. Alexander Mukasyan, for training, consultation, and/or experimental service.

Rates

Rates are updated annually.