Histology Core

THE HISTOLOGY CORE IS OPEN!!!

<PLEASE READ OUR REOPENING PLAN THOROUGHLY BEFORE REQUESTING SERVICES>

Histology Core (HC), NDIIF Operating Procedures during COVID-19 outbreak
Access to the Histology Core is subject to all NDIIF facility requirements. The following procedures are subject to change at any time. We sincerely appreciate your patience as we implement these changes.


REOPENING:
All dates below are tentative and subject to be change depending upon the guidance and leadership of UND. The cores will not be opened unless the building in which they reside in is open. If a building were to close unexpectedly, the core will also close during this time.


PHASE I – JUNE 8th- JUNE 10th
General rules: working with customers


1. All Histology training activities are suspended until further notice.
2. During this period, no users will be permitted in the facility while instruments and facility spaces are being prepared for reopening.
3. All communication will take place by appointment via email, telephone or through online conference tools such as Zoom, Skype, Microsoft Teams or WebEx.


PHASE II – June 10th – TBD following guidelines provided by UND
Disinfection will be performed at the beginning of each day by NDIIF staff. The hours of the Histology Core will remain 8:00am-5:00pm EST Monday thru Friday to coincide with the FLSC in which it is nested within.


1. Users will be permitted in the facility by appointment only during this period. A Certificate of Wellness must be submitted to the building manager at the building designated area immediately upon arrival into the building.


• DO NOT ACCESS THE FACILITY FOR A MINIMUM OF 14 DAYS if you have been in high risk areas or had contact with confirmed COVID-19 patients.
• DO NOT ACCESS THE FACILITY if you are COVID-19 positive or otherwise sick - even if you are only experiencing mild, cold-like symptoms.
If you are experiencing symptoms or have questions regarding COVID-19, you are encouraged to call the Notre Dame hotline: 574-631-5900. https://coronavirus.nd.edu/
• If you are at risk to develop severe symptoms, you are advised to avoid the facility and have colleagues or NDIIF staff do the imaging for you.


2. Only trained users are permitted to use the facility and instruments.
3. All sessions must be scheduled by Sarah Chapman with a minimum of 24 hours’ notice.
4. A daily calendar will be posted outside of the histology room. If you have not been
given permission to use the instruments within the Histology Core and your name does
not appear on the calendar, you MUST NOT enter the room. Contact Sarah Chapman if
you believe that a mistake has been made on the calendar and to be granted permission
to enter the room.
5. All ‘in person’ training or meetings are suspended until further notice. All
communication will take place by appointment via email, telephone or through online
conference tools such as Zoom, Skype, Microsoft Teams or WebEx.


 RULES UPON ENTRY
a. Wear a mask (user provided) at all times.
b. A lab coat (user provided) is required at all times while in the facility areas.
c. Personnel will wash hands immediately upon entering into the Histology facility
space. Where sinks are not present, hand sanitizer will be supplied.
d. Gloves (facility provided) are required when entering the facility until departure.
A waste container will be provided at the facility exits for disposing gloves.
e. Minimize bringing any personal belongings into the research space. Only bring
with you what is essential to your research.
f. Follow instrument specific disinfection procedure at the start of your session.
Disinfecting solutions (following CDC guidance): ethanol (70% min) with a
contact time of 1-2 minutes should be used and has been provided to wipe down
the surfaces of countertops, door handles, light switches and
computer/keyboard equipment.
g. A log placed within each room must be completed indicating that cleaning
procedures have been completed by each user as well as the NDIIF staff.
h. “Check in” - Register at the entrance with your name and phone number.
Signage has been posted on each door that must be filled out before entering
the instrument rooms. Also the user should indicate that the room is occupied
by using the slider magnet posted on the outside of the door.
i. Only ONE person can be in the room at a time. The Histology Core shares this
room with FLSC thus, the NDIIF will minimize its users to accommodate social
distancing measures with the FLSC staff that may also be in the room at the
same time. If the NDIIF user must share the space with FLSC staff, a social
distance of 6 feet apart must be maintained at all times.
- Doors must be closed while systems are in use. Do not enter the room if you see more than
two people working there. The break between Histology sessions should be at least 30min.
j. Follow instrument specific disinfection procedure at the end of your session.
Disinfecting solutions (following CDC guidance): ethanol (70% min) with a
contact time of 1-2 minutes should be used and has been provided to wipe down
the surfaces of countertops, door handles, light switches and
computer/keyboard equipment.
k. This building is shared by multiple users. Please check the hall before exiting the
facility so that you do not accidentally walk into another person and so that social
distancing of a minimum of six feet can be maintained at all times.
l. “Check Out” - Remove your name and phone number when leaving.
m. Wash hands or use hand sanitizer before leaving the space.


7. Histology services may be provided by Sarah Chapman (sarah.chapman@nd.edu). All
services must be arranged in advance via email with as much information regarding the
experiment as possible.
8. Services provided by Sarah Chapman for users during this period will be charged the
‘assisted use’ rate.
9. If instrument issues arise during the session, please call the staff at the phone number
provided. If these issues cannot be resolved with instruction over the phone, you will be
asked to shut down the system responsibly. A staff member will work to restore
function when it is safe to do so and the system will again be made available for use.
10. Do not leave in the lab any possessions including samples. A user cannot stay in the
room after the appointment time expired.
11. All assisted instrument operations will be conducted by Sarah Chapman, while the client
presence will not be allowed.


PHASE III – ‘The New Normal’ until restrictions ease as directed by UND leadership.


1. Access to instruments by trained users will continue as described in Phase II.
2. Training will take place by appointment and will be by conducted via online training and
conference tools.
3. Labs are encouraged to nominate a single “super-user” to minimize traffic through the
facility.

- In case of Histology Core related emergency please contact:
Sarah Chapman [sarah.chapman@nd.edu, cell phone (574) 514-6774]

Instruments and Services

 

The Notre Dame Histology Core (NDHC) provides a means to examine biological processes  in mice , rats, frogs, zebra fish, fruit flies, sheep bone, and even human tissue by use of immunohistochemical techniques and pathology. The facility is located in Freimann Life Sciences and it is equipped with a Shandon Citadel Tissue Processor, Leica microtome, Tissue-Tek III embedding station, and a new Leica Cryostat with a special Tungsten blade attachment capable of slicing through bone.

Services include tissue fixation, processing, embedding, sectioning, and staining of paraffin or frozen tissue sections. Staining of tissue sections ranges from routine H&E’s (Hematoxylin-Eosin) to special stains demonstrating specific tissue structures. The Core offers immunohistochemical staining with antibodies supplied by investigators. The Histology core is also able to provide SEM and TEM sample preparation as a service for biological and soft materials.

Dr. Kaliney offers free histopathology consultation sessions on the 2nd and 4th Thursdays of each month in Harper Hall A231, from 1pm to 4pm.  More information regarding Dr. Kaliney and his area of expertise can be found here.
 
If you are interested in meeting with Dr. Kaliney, contact Joey Dreitzler (jdreitzl@nd.edu).

Staff

Researchers can be trained to use our devices, or services can be provided by experienced staff.  Contact the  assistant director of biological imaging, Sarah Chapman, for training, consultation, and/or experimental service.

Rates

Rates are updated annually.