Hematxylin and Eosin
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Xylene - 5 minutes
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Xylene - 5 minutes
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Xylene - 5 minutes
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100% ETOH - 2 minutes
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100% ETOH - 2 minutes
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100% ETOH - 2 minutes
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95% ETOH - 2 minutes
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70% ETOH - 2 minutes
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Distilled Water - 2 minutes*
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Hematoxylin - 3-5 minutes
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Distilled Water - Rinse x3
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Clarifier - 30 seconds (20 dips)
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Distilled Water - 2 minutes
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Scott’s Tap Water - 30 sec (20 dips)
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Distilled Water - 1 minute
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70% ETOH - 1 minute
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Eosin Y - 1 to 2 minutes
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100% ETOH - 1 minute
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100% ETOH - 1 minute**
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100% ETOH - 2 minutes
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100% ETOH - 2 minutes
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Xylene - 2 minutes
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Xylene - 2 minutes
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Xylene - 2 minutes
*Stop at this point if you are deparrifinizing for immune staining.
**Start here for dehydrating and cover slipping.