Hematxylin and Eosin

  1. Xylene - 5 minutes

  2. Xylene - 5 minutes

  3. Xylene - 5 minutes

  4. 100% ETOH - 2 minutes

  5. 100% ETOH - 2 minutes

  6. 100% ETOH - 2 minutes

  7. 95% ETOH - 2 minutes

  8. 70% ETOH - 2 minutes

  9. Distilled Water - 2 minutes*

  10. Hematoxylin - 3-5 minutes

  11. Distilled Water - Rinse x3

  12. Clarifier - 30 seconds (20 dips)

  13. Distilled Water - 2 minutes

  14. Scott’s Tap Water - 30 sec (20 dips)

  15. Distilled Water - 1 minute

  16. 70% ETOH - 1 minute

  17. Eosin Y - 1 to 2 minutes

  18. 100% ETOH - 1 minute

  19. 100% ETOH - 1 minute**

  20. 100% ETOH - 2 minutes

  21. 100% ETOH - 2 minutes

  22. Xylene - 2 minutes

  23. Xylene - 2 minutes

  24. Xylene - 2 minutes

*Stop at this point if you are deparrifinizing for immune staining.

**Start here for dehydrating and cover slipping.