Optical Microscopy Core

THE OPTICAL MICROSCOPY CORE IS OPEN!!!

<PLEASE READ OUR REOPENING PLAN THOROUGHLY BEFORE REQUESTING SERVICES>

Optical Microscopy Core (OMC), NDIIF Operating Procedures during COVID-19 outbreak
Access to the Optical Microscopy Core is subject to all NDIIF facility requirements. The following procedures are subject to change at any time. We sincerely appreciate your patience as we implement these changes.


REOPENING:
All dates below are tentative and subject to be change depending upon the guidance and leadership of UND. The cores will not be opened unless the building in which they reside in is open. If a building were to close unexpectedly, the core will also close during this time.


PHASE I – JUNE 8th- JUNE 10th
General rules: working with customers


1. All Optical Microscopy training activities are suspended until further notice.
2. During this period, no users will be permitted in the facility while instruments and facility spaces are being prepared for reopening.
3. All communication will take place by appointment via email, telephone or through online conference tools such as Zoom, Skype, Microsoft Teams or WebEx.


PHASE II – June 11th – TBD following guidelines provided by UND
Disinfection will be performed at the beginning of each day by NDIIF staff. The facility hours during Phase II have been restricted to 6:00am to 10:00pm EST Monday thru Friday.


1. Users will be permitted in the facility by appointment only during this period. A Certificate of Wellness must be submitted to the building manager at the building designated area immediately upon arrival into the building.


• DO NOT ACCESS THE FACILITY FOR A MINIMUM OF 14 DAYS if you have been in high risk areas or had contact with confirmed COVID-19 patients.
• DO NOT ACCESS THE FACILITY if you are COVID-19 positive or otherwise sick - even if you are only experiencing mild, cold-like symptoms.
If you are experiencing symptoms or have questions regarding COVID-19, you are encouraged to call the Notre Dame hotline: 574-631-5900. https://coronavirus.nd.edu/
• If you are at risk to develop severe symptoms, you are advised to avoid the facility and have colleagues or NDIIF staff do the imaging for you.


2. Only trained users are permitted to use the facility and instruments.
3. All sessions must be scheduled by Sara Cole with a minimum of 24 hours’ notice.
4. A daily calendar will be posted outside of each instrument room. If you have not been given permission to use the instrument and your name does not appear on the calendar, you MUST NOT enter the room. Contact Sara if you believe that a mistake has been made on the calendar and to be granted permission to enter the room.
5. All ‘in person’ training or meetings are suspended until further notice. All communication will take place by appointment via email, telephone or through online conference tools such as Zoom, Skype, Microsoft Teams or WebEx.


 RULES UPON ENTRY
a. Wear a mask (user provided) at all times.
b. Personnel will wash hands immediately upon entering the Optical Microscopy spaces. Where sinks are not present, hand sanitizer will be supplied.
c. Gloves (facility provided) are required when entering the facility until departure. A waste container will be provided at the facility exits for disposing gloves.
d. Minimize bringing any personal belongings into the research space. Only bring with you what is essential to your research.
e. Follow instrument specific disinfection procedure at the start of your session. Disinfecting solutions (following CDC guidance): ethanol (70% min) with a contact time of 1-2 minutes should be used and has been provided to wipe down the surfaces of countertops, door handles, light switches and computer/keyboard equipment.
f. A log placed within each room must be completed indicating that cleaning procedures have been completed by each user as well as the NDIIF staff.
g. “Check in” - Register at the entrance with your name and phone number. Signage has been posted on each door that must be filled out before entering the instrument rooms. Also the user should indicate that the room is occupied by using the slider magnet posted on the outside of the door.
h. Only ONE person in each of the microscope rooms at a time.
- Doors must be closed while systems are in use. Do not enter the room if you see somebody working there. The break between OM sessions should be at least 30min.
i. Follow instrument specific disinfection procedure at the end of your session. Disinfecting solutions (following CDC guidance): ethanol (70% min) with a contact time of 1-2 minutes should be used and has been provided to wipe down the surfaces of countertops, door handles, light switches and computer/keyboard equipment.
j. Optics should be wiped down with the provided sanitized optical lens wipes. DO NOT USE kim wipes on any of the objectives or lenses.
k. Cover keyboards with plastic wrap. At the end of the appointment remove plastic wrap from keyboards and discard, disinfect mouse, knobs and other touched surfaces with disinfecting wipes.
l. This space is shared by multiple users. Please check the hall before exiting the facility so that you do not accidentally walk into another person and so that social distancing of a minimum of six feet can be maintained at all times.
m. “Check Out” - Remove your name and phone number when leaving.
n. Wash hands or use hand sanitizer before leaving the space.


6. Imaging services may be provided by Sara Cole (scole7@und.edu). All services must be arranged in advance via email with as much information regarding the sample as possible.
7. Services provided to users by Sara Cole during this period will be charged the ‘assisted use’ rate.
8. If instrument issues arise during the session, please call the staff at the phone number provided. If these issues cannot be resolved with instruction over the phone, you will be asked to shut down the system responsibly. A staff member will work to restore function when it is safe to do so and the system will again be made available for use.
9. Do not leave in the lab any possessions including samples. A user cannot stay in the room after the appointment time expired.
10. All assisted instrument operations will be conducted by Sara Cole, while the client presence will not be allowed.


PHASE III – ‘The New Normal’ until restrictions ease as directed by UND leadership.


1. Access to instruments by trained users will continue as described in Phase II.
2. Training will take place by appointment and will be by conducted via online training and conference tools.
3. Labs are encouraged to nominate a single “super-user” to minimize traffic through the facility.


- In case of Optical Microscopy Core related emergency please contact
Sara Cole [scole7@nd.edu, cell phone (614) 589-1940] or
Sarah Chapman [sarah.chapman@nd.edu, cell phone (574) 514-6774]

Instruments and Services

The Optical Microscopy Core (OMC) provides researchers with the opportunity to acquire informative single images and three-dimensional reconstructions of fluorescently labeled cells and tissues, either fixed or live. Our devices can accommodate samples mounted on either slides or in Petri dishes. Depending on the device used, images can include up to four separate channels with emission spectra ranging from those of DAPI to far-red fluorophores. Confocal 101 information shared by Cincinnati Children's Confocal Imaging Core.

Staff

Researchers can be trained to use our devices in as little as two hours. For consultations, scheduling of training, or for contracted image acquisition, contact the lab manager, Sara Cole.

Rates

Rates are updated annually.

Applications

  • Genetically encoded fluorescent proteins
  • Antibodies
  • Fluorescent molecular probes
  • Single cells and multilayered tissues
  • Changes in tissue architecture
  • Time-lapse movies
  • Movement and growth of sub-cellular organelles and macromolecules
  • Fluorescent ionic gradient reporters
  • Imaging of fixed and living systems: bacteria, protists, Drosophila, Zebrafish, all cultured cell types
  • Transparent non-living samples