Nikon A1R-MP Laser Scanning Confocal Microscope
With both fixed and live samples, the Nikon A1R is capable of generating high-resolution confocal images in up to four separate channels. Z-series of images can be used to generate three-dimensional renderings of samples. It is also capable of preforming FRET (fluorescence resonant energy transfer) analysis. The multi-photon excitation modality of the Nikon A1R-MP is best suited for long-term live tissue scanning in deep tissue. For more information on using this instrument, view our policies and rates.
Refer to Confocal 101 Imaging Information shared by Cincinnati Children's Confocal Imaging Core for tutorials on image acquisition and NIS Elements Software.
Confocal Imaging of Rhodopsin - (Left Frame) Manan Dhingra, Kirsten Groody, Young Moon, and Kara Ryan, of the Introduction to Undergraduate Research course taught by Prof. Whaley, are imaging rhodopsin protein in the eyes of mosquitos. Slides were prepared with care in the Histology Core. (Right Frame) Alex Metoxen, undergraduate researcher in the O'Tousa Lab, is examining rophodopsin (green) migration to light sensitive photoreceptor membranes (red) in the eye of the fruit fly.
Images of a 3D cultured tumor taken by Amy Leliaert from the lab of Prof. Zach Schafer.
Deep imaging of live tissue - The infrared laser employed in this modality enables fluorescence imaging to be performed at increased tissue depths. The two-photon capablity allows imaging with multiple filter sets through the entire cyst to generate three-dimensional data sets of these clusters of tumor cells.