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Nikon N-STORM

Nikon N-STORM Super Resolution Microscope

The Nikon N-STORM (STochastic Optical Reconstruction Microscopy) is a super-resolution optical microscope system based on an inverted research stand (Eclipse Ti2-E / Ti-E). It uses single-molecule localization microscopy (SMLM) to bypass the diffraction limit, delivering a 10-fold improvement in resolution over standard optical systems.

Features & Specifications

  • Microscope Stand: Inverted Nikon Eclipse Ti2-E / Ti-E equipped with Perfect Focus System (PFS) for hardware-based drift correction during long acquisitions.

  • Spatial Resolution:

    • Lateral (XY): ~20nm.

    • Axial (Z): ~50nm (via astigmatic optics or double-helix phase masks).

  • Imaging Modalities: 2D/3D SMLM (STORM/dSTORM, PALM), Total Internal Reflection Fluorescence (TIRF), HiLo, FRAP, and widefield/confocal overlay.

  • Excitation Laser Lines: Multi-laser source with lines at 405 nm, 488 nm, 561 nm, and 647 nm.

  • High-NA Objectives:

    • CFI SR HP Apochromat TIRF 100x Oil (NA 1.49).

    • CFI SR HP Plan Apochromat Lambda S 100x Silicone (NA 1.45) for index-matched deep-tissue imaging.

  • Detection System: High-sensitivity EMCCD camera (e.g., Andor iXon Ultra 897) or sCMOS cameras for high frame-rate localization acquisition.

  • Field of View: Up to 80 µm × 80 µm in wide-view mode, with optimized intermediate zoom optics.

  • Sample Environment: Compatible with 35 mm glass-bottom dishes, #1.5H coverslips, multi-well chambers, and full environmental incubation (temperature, CO2, humidity).