Nikon CSU-X1 Spinning Disk System

The Nikon CSU-X1 is optimized for high-speed imaging of living cells expressing fluorescent proteins and/or stained with synthetic dyes. Combined wide-field illumination of the sample, fast image capture and reduced phototoxicity/photobleaching make this system ideal for live cell imaging but can also be used for fixed samples.

Features & Specifications

• 3D confocal imaging
• 4-color fluorescence imaging
• Live cell time lapse imaging
• Automated multi-position imaging
• Stimulation with 440nm or 630nm LEDs though brightfield light path

Objectives

• Plan Apo λ 10x / 0.45
• Plan Apo VC 20x/0.75
• Plan Apo λ 40x/0.95
• Plan Apo λ 40x/1.3 oil
• Plan Apo VC 100X/1.4
• Apo TIRF 100x/1.49 Oil

Equipment Details

• Nikon Ti Microscope (Inverted)
• Yokogawa CSU-X1 Spinning Disk Confocal
• Vortran 4-line laser launch
• Sutter Lambda XL
• Photometrics Prime95B sCMOS camera
• Andor DU897 EMCCD
• Sutter Lambda 10-3 emission filter wheel
• ASI motorized XY stage with Z piezo
• OkoLab Cage Incubator
• OkoLab CO2 Mixer

Light Source

• Vortran 4-line solid state laser launch

Excitation Wavelengths

• 405nm
• 488nm
• 561nm
• 640nm

Location

Harper Hall Room 103